USC SCAP-T Protocols

USC Ephys Internal and External Solution Protocols Version 1

Internal pipet solution

  1. K-gluconate 130 mM; KCl 2 mM; CaCl2 1 mM; MgATP 4 mM; GTP 0.3 mM; phosphocreatine 8 mM; HEPES 10 mM; EGTA 11 mM; pH 7.25 and 300 mOsm) containing RNase inhibitor (0.4 U/ml; RNase inhibitor, Clontech)
  2. K-gluconate 130 mM; KCl 2 mM; CaCl2 1 mM; MgATP 4 mM; GTP 0.3 mM; phosphocreatine 8 mM; HEPES 10 mM; EGTA 11 mM; pH 7.25 and 300 mOsm) containing RNase inhibitor (0.4 U/ml; RNase inhibitor, Clontech) and Lucifer Yellow (0.625 mg/ml; Invitrogen, USA)

 

External bath solution

  1. NaCl 124 mM; KCl 4 mM; NaHCO3 26 mM; Glucose 10 mM; CaCl2 2 mM; MgCl2 2 mM (Recipe June 2013- May 2014)
  2. NaCl 124 mM; KCl 2.5 mM; NaH2PO4 1.2 mM; NaHCO3 24 mM; HEPES 5 mM; Glucose 12.5 mM; MgSO4 2 mM; CaCl2 2 mM 
 

 

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